Todays is Tobias’ last day in the lab. As part of his Master studies in Molecular Life Sciences at the Humboldt-Universität zu Berlin, he did a 6 month internship (so called Projektmodul) in our group. Tobias worked on the purification of tagged mutant versions of the protein Cas9 from Streptococcus pyogenes which is a key enzyme of the famous CRISPR system.
We will miss him in our team and wish all the best for his further studies!
During the last years, hundreds of novel RNA-binding proteins (RBPs) have been detected in mammalian cell lines. Now, we mapped the mRNA-bound proteome of a complete organism: the yeast S. cerevisiae. Additionally, we determined the mRNA interactome of human hepatocytic cells (HuH-7).
Having comprehensive information on RBPs from a eukaryotic single cell organism and from mammalian cell lines, we defined a conserved core mRNA interactome of eukaryotic mRNA-binders. The core consists of many well-established RNA-binding proteins but also of many enzymes from diverse biochemical pathways and glycolysis emerges as hot-spot of conserved RBPs.
We also identified another interesting difference among some conserved eukaryotic RBPs in their overall RNA-binding architecture: throughout evolution, a steady increase in the repetition of short amino acid tripeptide motifs in unstructured regions of proteins could be observed.
Beckmann BM, Horos R, Fischer B, Castello A, Eichelbaum K, Alleaume A-M, Schwarzl T, Curk T, Foehr S, Huber W, Krijgsveld J & Hentze MW. The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs. Nature Communications (2015); 6:10127
Julie has joined the group as research technician.
We finally managed to move the lab to our designated place:
Campus Nord of the Humboldt-Universität zu Berlin
Haus18 3rd floor
Davide Figini will join the lab from beginning of 2015 to do his PhD here.
We are currently updating our website: some information might be missing and will be updated soon.
Erika will join the lab as a master student starting from October 2014.
Erika will conduct her Masters Thesis here by establishing a novel protocol for purification of RNA-protein complexes.
Due to reconstruction works in Haus 18 (the IRI building), we are currently hosted by the Department of Biophysics:
Invalidenstrasse 42 (Mittelbau)